Mycosphere

The following research work aims to find a biocontrol alternative against the nematode of the knot (Meloidogyne incognita), through the use of 7 genders of endophytic fungi associated with the cultivation of sacha inchi (Plukenetia spp); which the percentage of parasitism on eggs and juvenile stages (J2) and the percentage of hatching of M. incognita eggs submitted to fungal extracts of mycoendophytic sacha inchi. We used 20 treatments (4 strains of Clonostachys sp, 4 strains of Trichoderma sp, 4 strains of Pestalotiopsis sp, 3 strains of Xylaria sp, 2 strains of Penicillium sp, 2 strains of Phyalophora sp and 1 strain of Acremonium sp) and one control. For the parasitism test, 21 treatments and 3 replicates were used, inoculating 500 eggs of M. incognita / 50 g of sterile soil and conidia of endophytic fungi in 1 x 106 ufc.cc in 90 x 15 Petri dishes mm, performing 4 evaluations at 4; 8; 12 and 16 d.a.exp. (days after exposure), with T2 treatments (T2: XYL-E-27); T4 (T4: CLO-E-84) and T16 (T16: PEN-E-117) which showed better results with an index of 91.67%; 92% and 92.33% of parasitism during the 4 evaluations compared to the other treatments. To test the in vitro effect of fungal extracts on egg hatching of M. incognita, 21 treatments and 3 replicates were prepared, formulating 3 concentrations (50%, 70% and 90%) in 150 x 15 mm test tubes, and inoculating with 600 eggs of M. incognita, performing 4 evaluations at 4; 8; 12 and 16 d.a.imm (days after immersion) being the best concentration at 90% and having T15 (T15: PES-E-135) as the best result with a 5.67% M hatch rate of M. Incognita during the four evaluations compared to the other treatments.