Resistencia antimicrobiana en cepas de Staphylococcus aureus aisladas de Leopardus garleppi (Carnivora: Felidae) en Ecuador

Antimicrobial resistance (AMR) is a global issue, yet research on this topic in wildlife remains limited. In this study, we evaluated antimicrobial resistance in Staphylococcus aureus strains isolated from the mucous membranes of a northern pampas cat (Leopardus garleppi) at the Amaru Biopark in Cuenca, Ecuador. Samples were collected from the nasal, conjunctival, and oral mucosa, as well as from skin tissue, and compared with samples from other felines in the study area. We tested susceptibility to nine antibiotics and observed higher resistance to beta-lactams, particularly penicillin and amoxicillin. The results indicate that Leopardus garleppi not only faces health risks but may also act as a reservoir for resistant microorganisms, posing a zoonotic threat to staff at ex situ conservation centers and to those who frequently interact with wildlife. Furthermore, we emphasize the importance of adopting a “One Health” approach through preventive strategies that enable a rapid and effective response to emerging diseases that compromise the well-being of these animals.