Primary producer biomarker profiles of bulk carbon and nitrogen stable isotopes (SI), compound specific stable isotopes of carbon in essential amino acids (CSIA-EAA) and their fatty acid (FA) collected from the Beaufort Sea coastal lagoons,2021-2024

Within Stefansson Sound in Prudhoe Bay, AK various organic matter sources were collected to determine multiple biomarker baseline profiles (i.e., bulk carbon and nitrogen stable isotopes (SI), compound specific stable isotopes of carbon in essential amino acids (CSIA-EAA), fatty acids (FA)). Some organic matter sources were collected from Elson lagoon in Utqiaġvik, AK and Kaktovik and Jago lagoons in Kaktovik, AK to supplement low sample sizes in some organic matter source groups. Kelp, red algae, terrestrial plants, phytoplankton, and ice algae were collected in 2024 with some supplement samples collected in 2021 - 2023. Stable isotope values of δ13C and δ15N are reported as “del_13c” and “del_15n”, respectively. Individual fatty acids are reported as the percent relative to total fatty acids for 23 fatty acids: C11:0, C12:0, C14:0, C15:1, C15:0, C16:0, C16:1n7, C17:0, C17:1, C18:0, C18:1n9 trans, C18:2n6 cis, C18:1n7, C18:3n3, C20:0, C18:3n6, C20:4n6, C21:0, C22:0, C22:1n9, C23:0, C24:0, C22:6n3. Stable isotope values of δ13C are reported in the following essential amino acids: Valine (Val), Leucine (Leu), iLeu (isoleucine), Methionine (Met), Phenylalanine (Phe). Additionally, we used ice algal diatoms collected in the Arctic (landfast ice near Utqiaġvik, Alaska) and cultured in a laboratory setting at the University of Alaska Fairbanks to compare the CSIA-EAA fingerprints of field (composites) ice algal samples and isolate diatoms samples.