mm10 500 bp UTRome (2022.08)

This data comprises a specialized set of gene annotations for the mm10 genome derived from GENCODE vM25. In brief, all FASTQ samples in the Mouse Cell Atlas v1.1 were preprocessed through PEAR to assemble paired-end reads then trimmed of polyA tails. Assembled reads were mapped to the mm10 `genome_snp_tran` index of HISAT2, and coverage of cleavage sites was extracted on a per-cell-type level. After merging to the mode over a window of 30 nts, cleavage sites with 5 TPM or more in at least one cell type were identified as cleavage site candidates. Sites were filtered based on the following criteria: 1) presence in GENCODE; 2) presence in PolyASite at least 3 TPM; 3) low probability of internal priming (