Quantitative proteomic dataset of primary lung fibroblasts from idiopathic pulmonary fibrosis and control donors

Proteomic profiling was performed on primary lung fibroblasts from 10 idiopathic pulmonary fibrosis (IPF) patients and 10 control donors. Proteins were extracted using a urea-based lysis buffer, digested with trypsin using a filter-aided sample preparation (FASP) protocol, and analyzed by LC–MS/MS. Data acquisition was conducted in data-dependent mode on an Orbitrap Exploris 480 mass spectrometer. Raw data were processed using Proteome Discoverer™ (v3.1) with the UniProt Homo sapiens database, applying a false discovery rate (FDR) of