Supplemental Figures S1-S2

Supplemental Figure S1. Whole-mount RNA-fluorescence in situ hybridization detection of proopiomelanocortin (Pomc) and growth hormone (Gh) mRNA expression in the chicken pituitary from embryonic day (e)8 to e17. Tissue sections represent the mid-focal plane from Z-stack acquisition, generated by stitching tiled images taken at 20X magnification. Pomc-expressing cells are labeled in red, Gh-expressing cells in green, and nuclei are counterstained with DAPI (blue). Remaining biological replicates (rep) from Figure 1.Supplemental Figure S2. Whole-mount RNA-fluorescence in situ hybridization detection of proopiomelanocortin (Pomc) and growth hormone (Gh) mRNA expression in the chicken pituitary from embryonic day (e)8 to e17. Panels display the caudal (Ca), medial (Md), or cephalic (Ce) region of the pituitary. Images represent projections of all Z-stack sections from dorsal to ventral; each acquired at 40X magnification and 1.5 μm intervals. Pomc‑expressing cells are labeled in red, Gh‑expressing cells in green, and nuclei are counterstained with DAPI (blue). Scale bars represent 60 μm. Remaining biological replicates (rep) from Figure 2, including rep 2 (A) and rep 3 (B).