Supplementary figures S1-S9 JAPPL

Figure S1. Changes in neuromuscular function during the fatiguing task. Dashed lines represent mean responses with aligned data points representing individual responses. Maximal voluntary contraction (MVC, panel A), root mean square EMG during each MVC normalised relative to the MVC at Baseline (TA rmsMVC, panel B), dorsiflexors evoked potentials (TAtw, pot panel C) during both the control condition (Control, in red) and blood flow restriction (BFR, in blue). †Denotes a condition × time interaction; *Significantly different from Control. Values are expressed as changes from Baseline and shown as means ± SD for 14 participants.Figure S2. Changes in root mean square EMG signal of the tibialis anterior muscle during the fatiguing task, normalised relative to the root mean square during the MVC performed at Baseline (TA rmsEMG) for both the control condition (Control, in red) and blood flow restriction (BFR, in blue). Dashed lines represent participant mean responses with aligned data points representing individual responses. †Denotes a condition × time interaction; *Significantly different from Control. Values are shown as means ± SD for 14 participants.Figure S3. Changes in heart rate and perceptual responses during the fatiguing task. Dashed lines represent participant mean responses with aligned data points representing individual responses. Heart rate (HR, panel A), rating of perceived exertion (RPE, panel B) and perceived muscle pain (PAIN, panel C) during both the control condition (Control, in red) and blood flow restriction (BFR, in blue). †Denotes a condition × time interaction; *Significantly different from Control. Values are expressed as changes from Baseline and shown as means ± SD for 14 participants.Figure S4. Identified population of motor units from the tibialis anterior muscle before (Baseline) and after the fatiguing task (Post) for each trapezoidal ramp contraction intensity. Motor unit discharge rate (panel A-C), recruitment threshold (panel D-F) and de-recruitment threshold (G-I) both the control condition (Control, in red) and blood flow restriction (BFR, in blue). #Denotes a main effect of time; *Significantly different from Control. Values are reported for 13 participants.Figure S5. Motor unit response before (Baseline) and after the fatiguing task (Post) normalised in respect to the maximal voluntary contraction performed at Post for each trapezoidal ramp contraction intensity. Box plots showing motor unit recruitment threshold (panel A-C) and de-recruitment threshold (panel D-F) for control condition (Control, in red) and blood flow restriction (BFR, in blue). #Significantly different from Baseline. Values are reported for 13 participants.Figure S6. Identified MU from the tibialis anterior before (Baseline) and after the fatiguing task (Post) normalised in respect to the maximal voluntary contraction performed at Post for each trapezoidal ramp contraction intensity. Recruitment threshold (panel A-C), de-recruitment threshold (panel D-F) for the control condition (Control, in red) and blood flow restriction (BFR, in blue). #Significantly different from Baseline. Values are reported for 13 participants.Figure S7. Identified population of relatively lower-threshold motor units (LT MU) and relatively higher-threshold motor units (HT MU) of the tibialis anterior muscle from the tibialis muscle before (Baseline) and after the fatiguing task (Post). Motor unit discharge rate (panel A-B), recruitment threshold (panel C-D) and de-recruitment threshold (panel E-F) for control condition (Control, in red) and blood flow restriction (BFR, in blue). #Denotes a main effect of time; *Significantly different from Control. Values are reported for 13 participants.Figure S8. Motor unit response before (Baseline) and after the fatiguing task (Post) normalised in respect to the maximal voluntary contraction performed at Post. Box plots showing recruitment threshold (panel A-B) and de-recruitment threshold (panel C-D) for control condition (Control, in red) and blood flow restriction (BFR, in blue). Relatively lower-threshold motor units (LT MU). Relatively higher-threshold motor units (HT MU). #Denotes a main effect of time; #Significantly different from Baseline. Values are reported for 13 participants.Figure S9. Identified MU from the tibialis anterior muscle before (Baseline) and after the fatiguing task (Post) normalised in respect to the maximal voluntary contraction performed at Post. Recruitment threshold (panel A-B) and de-recruitment threshold (panel C-D) both for control condition (Control, in red) and blood flow restriction (BFR, in blue). Relatively lower-threshold motor units (LT MU). Relatively higher-threshold motor units (HT MU). #Denotes a main effect of time; #Significantly different from Baseline. Values are reported for 13 participants.