Figure 3H-I primary data

Figure 3H-I BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”). Where indicated, the cells were also treated with 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”) and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. MxA mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. In H, MxA mRNA levels are plotted relative to the lytic+casp-i+anti-IFN Abs sample. The lytic+casp-i+anti-IFN Abs in I samples were sorted based on tdTomato expression and the fold enrichment in MxA mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale.