Supplementary Figures Nencini et al

The document contains supplemental materials for a scientific study involving bisphenol A (BPA) exposure, oxidative stress, and small extracellular vesicles (sEVs) derived from human placental explants, U-373 MG cells, and HBMEC cells.The file includes:1. Supplemental Figure 1A graphical summary of the experimental workflow:A) Human placental explants cultured under control or BPA (1 nM) conditions for 48 h, followed by sEV isolation and tissue analysis.B) U-373 MG cells treated with BPA (10 nM), BPA + BPA-placenta sEVs, or BPA + BPA-HBMEC sEVs. Main outcomes: ROS formation, viability, mitochondrial activation, antioxidant gene expression.2. Supplemental Figure 2Characterization of sEVs from HBMEC cells and their effects on oxidative status:A) Nanosight particle analysis of control vs BPA-exposed sEVs.B) Western blots showing IRE1α, TSG101, CD81 expression in sEVs.C) ROS formation in U-373 MG cells under different treatments.3. Supplemental Table 1A table listing information on qPCR primers used in the study (Bio-Rad assays). Although primer sequences are not disclosed by the manufacturer, the table includes:Gene symbolGene nameRefSeq accession numberAmplicon size (~100 bp for all)Assay type (all SYBR Green)