Fig 5. Deletion of the N-terminal domain of MYSM1 enhances early inflammatory response in secondary infections in septic mice.

(A) According to the schematic diagram of the animal model 1, WT and MYSM1ex2-Δ/Δ mice were intraperitoneally injected with LPS solution and E. coli suspension (3 × 108 CFU/mL). Tissue collection and analysis were performed 4 hours post-injection; Fig 5A: Citation to Use: Created in BioRender. An, Q. (2026) https://BioRender.com/68gegzc. (B) Peritoneal lavage fluid was collected from WT (n = 3) and MYSM1ex2-Δ/Δ mice (n = 3), and the mRNA expression levels of IL-1β and IL-6 were determined by RT-PCR. (C) Lung tissues were collected from WT (n = 4) and MYSM1ex2-Δ/Δ mice (n = 4). The protein expression of F4/80, LY6G, and IL-1β in the lungs was examined by immunohistochemical analysis, and the histological features were evaluated using hematoxylin and eosin (H&E) staining. (D) Spleen tissues were collected from WT (n = 4) and MYSM1ex2-Δ/Δ mice (n = 4),The protein expression of F4/80 and IL-1β in the spleen was examined by immunohistochemical analysis, and the histological morphology was analyzed using hematoxylin and eosin (H&E) staining. The data were expressed as means ± SDs. The statistical analysis was carried out using two-sided Student’s t-test (B, C). The data were considered statistically significant when *p ≤ 0.05, **p ≤ 0.01,***p ≤ 0.001, and ****p ≤ 0.0001. https://doi.org/10.6084/m9.figshare.30581516.