Fig 6. Deletion of the N-terminal domain of MYSM1 improves late survival and prognosis after secondary infection in septic mice.

(A) Experimental mice were intraperitoneally injected according to the schematic diagram of the animal model 2. Subsequently, the spleens were isolated, and morphological observations were conducted and photographed; Fig 6A: Citation to Use: Created in BioRender. An, Q. (2026) https://BioRender.com/z5mdalr. (B) Spleens were isolated and weighed from the LPS pretreatment + E. coli infection group of WT (n = 8) and MYSM1ex2-Δ/Δ (n = 11) mice, and the spleen index was analyzed for WT and MYSM1ex2-Δ/Δ mice; Fig 6B: Citation to Use: Created in BioRender. An, Q. (2026) https://BioRender.com/4kjxje4. (C) Survival curves were recorded and analyzed for the LPS pretreatment + E. coli infection group of WT (n = 12) and MYSM1ex2-Δ/Δ (n = 12) mice; Fig 6C: Citation to Use: Created in BioRender. Xjl, X. (2026) https://BioRender.com/okvc1xv. (D) Survival curve of WT (n = 12) and MYSM1ex2-Δ/Δ (n = 12) mice pretreated with PRT4165 (20 mg/kg, once daily for 3 consecutive days) prior to intraperitoneal challenge with LPS or E. coli. Fig 6D: Citation to Use: Created in BioRender. An, Q. (2026) https://BioRender.com/kxjpyt0. (E) Bacterial load in the lung tissues was measured for the LPS pretreatment + E. coli infection group of WT (n = 4) and MYSM1ex2-Δ/Δ (n = 4) mice. (F) Protein levels of F4/80, LY6G, and IL-1β in the lungs of the LPS pretreatment + E. coli infection group of WT (n = 4) and MYSM1ex2-Δ/Δ (n = 4) mice were determined by immunohistochemical analysis. Histological features of the lung tissues were analyzed using hematoxylin and eosin (H&E) staining. The data were expressed as means ± SDs. Statistical analysis was performed using two-sided Student’s t-test (B, E, F) or Kaplan–Meier survival analysis (C, D). The data were considered statistically significant when *p ≤ 0.05, **p ≤ 0.01,***p ≤ 0.001, and ****p ≤ 0.0001. https://doi.org/10.6084/m9.figshare.30581531.