S2 Fig. LPS-induced immunosuppression in BMDMs.

(A, B) Bone marrow–derived macrophages (BMDMs) were isolated from WT mice and stimulated with LPS for 6, 12, or 24 hours. IL-1β and IL-6 mRNA levels were quantified by RT-PCR; (C, D) To model secondary infection in sepsis, BMDMs were stimulated with LPS, and IL-1β and IL-6 mRNA expression was measured by RT-PCR. The data are expressed as means ± SDs. Statistical analysis was carried out using one-way ANOVA. The datawereconsidered statistically significant when P ≤ 0.01 (**). https://doi.org/10.6084/m9.figshare.30581567.