S5 Fig. Phenotypic characteristics of MYSM1ex2-Δ/Δ mice.

(A) Schematic diagram of the CRISPR/Cas9-based design strategy for generating MYSM1ex2-Δ/Δ mice. S5A Fig: Citation to Use: Created in BioRender. An, Q. (2026) https://BioRender.com/7eganov. (B) MYSM1 protein levels in peritoneal macrophages from WT and MYSM1ex2-Δ/Δ mice were detected by WB. (C) In the LPS-induced secondary infection cell model of sepsis usingPMsderived from WT and MYSM1ex2-Δ/Δ mice, P-P65 protein levels were detected by WB. (D) Representative gross appearance of WT and MYSM1ex2-Δ/Δ mice. (E) Body weight of WT(n = 12) and MYSM1ex2-Δ/Δ mice(n = 12). (F) In the LPS-induced secondary infection cell model of sepsis usingPMsderived from WT and MYSM1ex2-Δ/Δ mice, immunoprecipitation (Co-IP) and western blot (WB) analysis were performed to validate the specificity of the H2AK119ub antibody. https://doi.org/10.6084/m9.figshare.30581600.