Figure S1: CF iPSC-airway shows elevated susceptibility to SARS-CoV-2 infection and greater epithelial damage, which is reduced by highly effective CFTR-modulator

A) Immunofluorescence analysis of mock infected CF and CFTR-corrected iPSC-airway at 1 dpi stained for viral nucleocapsid (SARS-CoV-2 N, green) and α-tubulin (red) at 1 and 3 dpi. Nuclei are stained with DAPI (blue). Scale bar = 20 μmB) Percentage of SARS-CoV-2 N positive cells by immunoimaging, expressed as a percentage of total DAPI+ positive cells.C) RT-qPCR of mRNA of the SARS-CoV-2 entry factors ACE2 and TMPRSS2 in CF and CFTR-corrected iPSC airway (n=9). Closed, open circles and triangles indicate 3 separate experiments.D) Representative images of RNA-scope of CF and CFTR-corrected iPSC airway cultures. Purple = FISH of ACE2 (top) and TMPRSS2 (bottom).