Multi-omics Alterations Associated with Tolerance and Response to Simulated Progressive Hemorrhage in Healthy Adult Humans

Figure S1: Longitudinal changes in plasma protein abundance following LBNP. Changes in Normalized Protein Expression (NPX) for top significantly regulated proteins measured via Olink proteomic panels. Data represents all study participants (N=133) at Pre- and Post-LBNP timepoints. Points and vertical bars indicate the Mean ± Standard Deviation (SD). Red lines represent the High Tolerance (HT, n=90) group, and blue lines represent the Low Tolerance (LT, n=43) group.Figure S2. Genome-wide association analysis of LBNP tolerance. Manhattan plot derived from whole-exome sequencing of the USAISR cohort (n=46). The X-axis displays chromosomal position, and the Y-axis represents the statistical significance (-log10 P-value) of the association between genetic variants and the Cumulative Stress Index (CSI). The top dashed horizontal line indicates the genome-wide significance threshold (P < 5E-8), and the bottom dashed line indicates the suggestive significance threshold (P < 1E-5). Labeled points denote variants in specific genes of interest that passed the suggestive significance threshold.Figure S3. Lower Body Negative Pressure (LBNP) experiment pressure profile over time. Schematic representation of the stepwise LBNP protocol used to simulate progressive hemorrhage. The X-axis represents time in minutes, and the Y-axis represents chamber pressure in mmHg. The protocol consists of a 5-minute baseline (0 mmHg), followed by stepwise decompression to -15, -30, -45, and -60 mmHg, with subsequent -10 mmHg increments every 5 minutes until the onset of hemodynamic decompensation.