EGF vs Oligo‑215: Calcium Signaling in PC12 Cells After Laser‑Induced Shockwave

This study investigates how EGF and Oligopeptide‑215 influence calcium signaling in PC12 cells following mechanical injury induced by Laser‑Induced Shockwave (LIS). PC12 cells were loaded with Fluo‑4 AM and ethidium dimer III to track calcium dynamics and exclude non‑viable cells. Treatments included 110 ppm Oligo‑215, 110 ng/mL EGF, and corresponding solvent controls. Live-cell imaging captured calcium responses every 3 seconds for 30 minutes, with LIS applied at the 3‑minute mark. Calcium transients were quantified as F/F₀, and statistical analysis using one‑way ANOVA with post hoc comparisons revealed no significant difference in peak calcium responses between Oligo‑215 + SW and EGF + SW groups. These results indicate that Oligo‑215 does not alter the magnitude of shockwave‑evoked calcium increases compared to EGF. Since calcium transients represent early intracellular activation, the comparable responses suggest that Oligo‑215 may engage pathways parallel to EGF during injury‑related stimulation. Future work will examine downstream signaling and potential effects on cell survival.