Additional file 1 of Microgravity activates monocyte ERK1/2 signaling and modulates the response to lipopolysaccharide

Supplementary Material 1: Supplementary Figure 1. Fluorescenceimages of human monocytes under 1G and microgravity (µG) conditions, with andwithout LPS stimulation. Green: cytoplasmic MAPK activity/viability (anti-pERK antibody); Blue nuclei (DAPI). Localized regions of high fluorescence indicate activated cells; quantitative clustering was not measured. 1G + LPS shows areas of concentrated signal, while µG and µG + LPS show more scattered activation patterns, consistent with reduced stimulus responsiveness under microgravity.