Additional file 1 of Development of a field-deployable RPA-CRISPR/Cas12a assay for the detection of Cyclospora cayetanensis in human feces

Additional file 1: Figure S1. Homemade DNA banding column. Figure S2. Gel electrophoresis of annealing, transcription and purified products. Figure S3. Result of PCR amplification based on the Cox3 locus of C. cayetanensis. Figure S4. Evaluation of RPA primers by agarose gel electrophoresis. Figure S5. Select Target sequence. Figure S6. The real-time fluorescence intensity curve of the nucleic acid sensitivity test based on the fluorescence detection of RECCT-Cay. Figure S7. The real-time fluorescence intensity curve of the oocyst sensitivity test based on the fluorescence detection of RECCT-Cay. Figure S8. The real-time fluorescence intensity curve of the specificity test of the RPA-CRISPR/Cas12a-based fluorescence detection. Figure S9. Results of C. cayetanensis amplification in human fecal samples based on 18S rRNA gene locus. Figure S10. Images of RPA and CRISPR/Cas12a lyophilized reagents in an eight-row tube tubes. Figure S11. Evaluating the feasibility of a portable suitcase test using a positive stool sample for C. cayetanensis. Table S1. Nucleotide sequences used in this study. Table S2. Information of non-target parasite DNA samples. Table S3. Information of 30 fecal clinical samples from inpatients. Table S4. Concentration determination of purified crRNA.