Additional file 1 of CD248, targeted by veratramine and neobavaisoflavone, mediates pathological changes of renal tubular epithelial cells induced by high glucose

Supplementary Material 1. Supplementary figure 1. Activation of TGF-β1 reverses the protective effects of CD248 knockdown on HG-induced injury in HK-2 cells. HK-2 cells were treated under normal glucose (NG) or high glucose (HG) conditions, transfected with si-CD248#1, and/or stimulated with the TGF-β1 activator SRI-011381. A. The protein expression levels of TGF-β1, p-Smad2, p-Smad3, Smad4 were detected by Western blot. B. Cell viability was detected by CCK-8 assay. C. Cell apoptosis was detected by flow cytometry. D. The mRNA expression levels of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) were detected by qPCR. E-H. Then the protein expression levels of EMT-related markers (E-cadherin, Vimentin and α-SMA) (E&G) and ECM proteins (collagen I, collagen IV and fibronectin) (F&H) were detected by Western blot. Data were presented as mean ± SD (n = 3 independent experiments). *P