Additional file 2 of CD248, targeted by veratramine and neobavaisoflavone, mediates pathological changes of renal tubular epithelial cells induced by high glucose

Supplementary Material 2. Supplementary figure 2. VER and NBIF bind directly to CD248 protein in HK-2 cells, as demonstrated by CETSA. A&B. HK-2 cells were treated with DMSO, 20 μM VER (A), or 40 μM NBIF (B) for 4 h. Cell lysates were heated to the indicated temperatures and soluble CD248 protein was detected by Western blot. Data were presented as mean ± SD (n = 3 independent experiments). *P