Additional file 3 of CD248, targeted by veratramine and neobavaisoflavone, mediates pathological changes of renal tubular epithelial cells induced by high glucose

Supplementary Material 3. Supplementary figure 3. VER and NBIF ameliorate HG-induced injury in HK-2 cells through both CD248-dependent and independent mechanisms. HK-2 cells were transfected with si-NC or si-CD248 for 48 h, followed by pretreatment with 20 μM VER, 40 μM NBIF, or DMSO for 24 h, and then stimulated with high glucose (HG) for another 48 h. A. Cell viability was detected by CCK-8 assay. B. Cell apoptosis was detected by flow cytometry. Data are presented as mean ± SD (n = 3 independent experiments). **P < 0.05, ***P< 0.001.