Characterisation and bioactivity of sulphated exopolysaccharide produce by Halomonas titanicae PCRB123b from Antarctic Ocean

This study investigates sulphated exopolysaccharide (EPS) produced by Halomonas titanicae PCRB123b, isolated from the polyextreme Antarctic Ocean. The EPS has molecular weight ∼1.48 × 104 Da. Surface topography revealed smooth, non-porous “weaving flasks” configuration. The CHNS data confirmed high carbon (21.41%) and sulphur (8.99%) content in EPS. Monosaccharide analysis bares a heteropolysaccharide configuration with mannose (82.9%), glucose (8.85%), and xylose (2.25%) etc. NMR and FTIR spectroscopy confirmed →4)-β-D-Manp-(1→ and →4)-β-D-Glcp-(1→ glycosidic linkages of mannose with glucose monomers, and xylose linked with mannose by →3)-β-D-xylp-(1→ glycosidic linkage. EPS was stable up-to >300 °C and exhibited a strong antioxidant activity against several free radicals. It has excellent emulsification properties for different food oils and strong flocculation bioactivity rate of 98.23% at 15 mg/L concentration, also 342.5% water and 185% of oil holding capacity. These findings suggest that PCRB123b EPS could be a beneficial addition to the food processing industry, potentially replacing synthetic polymers.