Additional file 2 of PCR-based detection and pathogenicity assessment of Stilbocrea banihashemiana in pomegranate orchards

Additional file 2：Figure S1. Morphological and cultural characteristics of S. banihashemiana isolated from symptomatic pomegranate trees in Fars Province, Iran. Figure S2. Detached pomegranate shoots inoculated with 26 recovered S. banihashemiana isolates are shown, observed 15 days post-inoculation. Figure S3. A heatmap illustrating the severity of three pathogenicity traits, including upward lesion progression (ULP), downward lesion progression (DLP), and lesion width (LW), on inoculated pomegranate detached shoots with 26 S. banihashemiana isolates. Figure S4. The progression of disease symptoms, including decline, yellowing, and defoliation, in three pomegranate cultivars— a ‘Rabab-e-Neyriz’, b ‘Kadro’, and c ‘Bihaste Ravar’—three months post-inoculation. d For comparison, the negative control shows saplings inoculated with a non-colonized PDA plug, which remain healthy with no observable symptoms. Figure S5. A heatmap illustrating the severity of six pathogenicity traits, including lesion length (LL), lesion width (LW), internal lesion length (ILL), vascular progression (VP), lesion depth (LD), and number of days with symptoms (NDS), evaluated over three months (90 days minus incubation period days) on saplings from seven pomegranate cultivars. Figure S6. Species-specific PCR detection of S. banihashemiana in wood samples from seven inoculated pomegranate cultivars.