Stability indicating RP-HPLC method for the simultaneous determination of multi-flavonoid compounds in herbal extracts, besides green analytics

A stability-indicating RP-HPLC method was developed and validated for the simultaneous quantification of five flavonoids, viz., epigallocatechin gallate (EGCG), silibinin (SL), naringenin (NG), genistein (GT), and apigenin (AG), in herbal extracts. Chromatographic separation was achieved on a C18 column using 0.1 M ammonium acetate and methanol at 40:60% v/v. The method demonstrated acceptable resolution, linearity (r2: 0.9905–0.9969), precision (98%. The method demonstrated acceptable green chemistry by the AGREES and GAPI tools.