Supplementary Figure 1 for "Auditory Encoding Regulated by the Dorsal Hippocampus CA1: Direct Evidence from the Lateral Entorhinal Cortex"

Supplementary Figure 1. Silencing the dCA1–LEC circuit selectively impairs memory without affecting locomotion or anxiety-like behavior. (A) Schematic illustrating the viral injection strategy in dCA1 and cannula implantation for drug delivery in LEC. (B) Representative image showing viral expression (mCherry) at the injection site in dCA1 (Scale bar: 1000 μm). (C) Open field test (OFT) results. Left: Total distance traveled in 5 minutes. Right: Time spent in the center zone (two-sample t-test, t₂₂ = 1.919, P = 0.068). Data are presented as mean ± SEM. N = 12 mice for each group. (D) Immobility time during the forced swim test (two-sample t-test, t₂₂ = 0.992, P = 0.332). N = 12 mice for each group. (E) Elevated plus maze (EPM) results. Left: Number of entries into open arms. Right: Percentage of time spent in open arms (two-sample t-test, entries: t₂₂ = 0.456, P = 0.653; time: t₂₂ = 0.635, P = 0.532). N = 12 mice for each group. (F) Experimental timeline for auditory fear conditioning (training) and memory retrieval (testing). N = 12 mice for each group. (G) Freezing levels (%) during the six training trials for representative control and experimental (dCA1–LEC silenced) animals. (H) Freezing levels during the testing phase. Left: Pre-conditioned stimulus (Pre-CS) baseline. Right: During conditioned stimulus (CS) presentation (two-sample t-test, Pre-CS: t₂₂ = 1.276, P = 0.215; CS: t₂₂ = 3.514, P = 0.002). N = 12 mice for each group. (I) Experimental timeline for the Novel Object Recognition test. (J) Exploration time for the two identical objects during the training phase (paired t-test, Control: t₁₁ = 0.127, P = 0.901; CNO: t₁₁ = 0.506, P = 0.623). N = 12 mice for each group. (K) Exploration time for the familiar versus novel object during the testing phase (paired t-test, Control: t₁₁ = 5.987, P < 0.001; CNO: t₁₁ = 0.134, P = 0.896). N = 12 mice for each group.