S1E Figure

S1 Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORF57 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. Immediate early genes: ORF50 (A-B), ORFK5 (C-D). The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 1 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p>0.05, ** = p