S3B Figure

S3B Fig. Mitochondrial DNA is the likely cGAS ligand during lytic KSHV infection. BC-3 cells were treated with or without 20 μM dideoxycytidine (“ddC”) for 48 hours to deplete mitochondrial DNA (“mtDNA”), then treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), or vehicle (DMSO, “latent”). At the time of lytic induction, where indicated, the cells were also treated with 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”). RNA was extracted and IFN-β mRNA levels were measured by RT-qPCR, normalized to 18S rRNA, and plotted relative to the lytic+casp-i sample. ns = p>0.05, ** = p