Algorithm to increase the maximum count rate of nanoparticles measured by flow cytometry

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Authors: Mona Shahsavari, Edwin van der Pol, Ton G. J. M. van Leeuwen, Arjan Wiskerke
Cited by: 0

Abstract

A pulse fitting algorithm was developed and validated to extend the operating range of flow cytometry at high particle concentrations. Tested on polystyrene beads and Intralipid samples, the algorithm enabled accurate quantification up to 3.0E+5 to 4.6E+5 events per second, representing a 10- to 49-fold improvement over conventional electronics.

Fields of Study & Subjects

Microfluidic and Bio-sensing Technologies Advanced Measurement and Detection Methods Advanced Fluorescence Microscopy Techniques

Publication details

Journal / source
Figshare
Publication date
29 January 2026
Document type
Dataset