Additional file 1 of Macrophages and iNOS contribute to the effects of dural prolactin and repeated stress in mouse migraine models

Supplementary Material 1: Supplementary Fig. 1 Depletion of peritoneal macrophages using clodronate liposome injection. (A) Mice received two i.p. injections of 150 µl clodronate liposomes (Clod. Liposomes; selective depletion of macrophages) or two i.p. injections of 150 µl of control liposomes (Ctrl. Liposomes) at 48-hours interval. Flow cytometric quantification of peritoneal macrophages 24 h after second injection of clodronate liposomes or control liposomes (n = 2 per group). (B) Representative flow cytometry plots of macrophages (CD45⁺F4/80⁺ live cells) in the peritoneal cavity of mice treated with clodronate liposomes. (C) Representative flow cytometry plots of macrophages (CD45⁺F4/80⁺ live cells) in the peritoneal cavity of mice treated with control liposomes. * indicates Clodronate Liposomes vs. Ctrl. Liposomes. Significant differences were determined through Unpaired t-tests. Data are represented as mean ± SEM. **