Additional file 1 of Divergent functions of three Kunitz trypsin inhibitor (KTI) proteins in herbivore defense in poplar

Additional file 1: Additional Figure S1: Phylogenetic tree of putative Kunitz Trypsin Inhibitors in Populus trichocarpa and the haplotype sequences of P. tremula and P. alba. Additional Figure S2: Multiple sequence alignment of amino acids of the three candidate Kunitz Trypsin Inhibitor proteins. Additional Figure S3: Linear curves for primer efficiencies. Additional Figure S4: a) KTI transcript levels of P. x canescens plantlets grown and wounded under sterile conditions, b) KTI (Potri019G08220, KTI_8220) transcript levels of greenhouse grown poplars after exposure to methyl-jasmonate (meJA), ACC or BTH. Additional Figure S5: Transcript abundances of KTI_400, KTI_600 and KTI_53200 over-expressed under the 35S promoter. Additional Figure S6: Twelve-week-old Kunitz Trypsin Inhibitor mutant lines grown under greenhouse conditions. Additional Figure S7: Representative photographs of Kunitz Trypsin Inhibitor poplar mutant lines under constant feeding of Helicoverpa armigera larvae. Additional Table S1: Data set for potential KTIs extracted from Kaling et al. 2018. Additional Table S2: List of all primer sets used for cloning and standard PCR. Additional Table S3: List of primers for RT-qPCR. Additional Table S4: Gene identity numbers for P. trichocarpa (Potri) and P. tremula (Potra). Additional Table S5: Subcellular localization of the candidate KTIs. Additional Table S6: Description of transformed and surviving mutant lines of Kunitz Trypsin Inhibitor in Populus x canescens. Additional Table S7: Consequences of CRISPR-Cas12a editing events observed in mutant lines. Additional Table S8: Gas exchange and growth of wildtype and transgenic poplar lines.