Clone-based fungal mock community enables strain-level discrimination for Oxford Nanopore taxonomic profiling

Figshare 2026 Dataset Open access

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Authors: Yin-Tse Huang
DOI: 10.6084/m9.figshare.31162168
Cited by: 0

Abstract

We developed a clone-based fungal mock community comprising 22 isolates, including dikaryotic pairs for strain-level discrimination testing. We systematically benchmarked four Oxford Nanopore taxonomic profiling methods -- Emu, Kraken2/Bracken, NanoCLUST, and AmpliconSorter -- across datasets using a dual-level assessment framework evaluating both species detection and strain resolution

Fields of Study & Subjects

Genomics and Phylogenetic Studies Mycorrhizal Fungi and Plant Interactions Environmental DNA in Biodiversity Studies

Publication details

Journal / source
Figshare
Publication date
1 January 2026
Document type
Dataset