Additional file 1 of Comparative quantification of Leishmania infantum in experimental phlebotomine sand fly infections using kDNA and single-copy Meta-1 gene qPCR assays

Supplementary material 1: Fig. S1. Melting curve analyses showing the melting temperature (Tm) peaks of the qPCR standard series prepared from a primary L. infantum ARIS DNA extract (106 parasites/mL) with five 1:10 serial dilutions; replicates show a single sharp peak at ~85 °C, indicating a specific amplicon with no nonspecific products or primerdimers.