Additional file 5 of c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT

Supplementary Material 5. Supplementary Figure 5. A) CUT&RUN and RNAseq tracks displaying the expression levels of the Snai1, Snai2, Tgfb1, Zeb2, Itga5 and Itgav genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B) Pearson correlations of REL with SNAI1, SNAI2, TGFB1, ZEB2, ITGA% and ITGAV mRNAs in the TCGA cohort. The graphs are adapted from the UCSC-Xena database. C) Kaplan-Meier survival curves for ITGA5 and ITGAV expression in the TCGA cohort. Samples are divided from median expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. D) Correlation of c-Rel expression with Itgβ3, cancer cell FN1 and stromal FN1 expression in mouse CKP tumors. Representative staining images from the TMAs are given. E) Correlation of c-Rel versus ITGA5 H-scores in patient TMAs on the left plot. On the right, overall survival curve for the ITGA5 H-score given. F) Average patient TMA H-scores are analyzed in samples divided based on various clinical parameters. G) Heatmap displaying DEGs whose promoters are also occupied by c-Rel in degron cells (up- or downregulated). H) Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that are differentially expressed and downregulated in Rel-pAY15 cells compared with those in pAY15 only or Rel-pAY15 cells induced with 5phIAA. From each set, only the top 5 hits are selected on the basis of their combined scores.