Additional file 1 of Integrin activation by two independently regulated calcium-mediated pathways is required for neutrophil recruitment

Supplementary Material 1: Supplemental Fig. 1 Expression of Ca2+ isoforms in neutrophils. Determination of Ca2+ isoforms via immunoblotting from control and (A-B) STIM1-KO = STIM1LysM-Cre+, (C-D) ORAI1-KO =ORAI1LysM-Cre+, (E-G) STIM1/2-KO = STIM1/2LysM-Cre+, (H-J) STIM1/ORAI1-KO = STIM1/ORAI1LysM-Cre+, (K-L) STIM2-KO = STIM2LysM-Cre+ and (M-N) ORAI2-KO = ORAI2LysM-Cre+ neutrophils. Lysates were immunoblotted with (A, E, H) anti-STIM1, (C, H) anti-ORAI1, (E, K) anti-STIM2, (M) anti-ORAI2 and (A, C, E, H, K, M) anti-GAPDH, n=3, experimental repeat. Representative Western blot images are cropped. Data are mean ± SEM. *p