Additional file 1 of Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid

Supplementary Figure 1: Validation of the self-aggregation characteristics of different rec PrP substrates. Five reactions without CSF seed (red line), five without substrate (blue line), and five with non-prion CSF controls (green line) using chimeric hamster–sheep (A), FL Hu (B), and FL Hu E200K (C) substrates showed a negative seeding response (flat lines) after 80 h of measurement. RT-QuIC reactions using the rec PrP FL Hu D178N (D) substrate, either seeded with control CSF samples from non-prion disease cases (green line) or without CSF seed (red line), indicated self-aggregation properties of this substrate.