Additional file 2 of Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid

Supplementary Figure 2: RT-QuIC reactions with chimeric hamster-sheep, FL Hu, and FL Hu E200K substrates across various freezing and thawing cycles. Mean fluorescence values of RT-QuIC assay with chimeric hamster-sheep (A1), FL Hu (B1), and FL Hu E200K (C1) substrates seeded with sCJD CSF (n = 5) after different cycles of freezing and thawing (T = 0, 4, 8 and 12). Neither the chimeric hamster-sheep substrate (A), the FL Hu (B), nor the FL Hu E200K (C) substrate reactions revealed significantly different signal responses after 12 freezing and thawing cycles in the area under the curve (AUC) (A2, B2 and C2) and lag-phase (A3, B3 and C3)