Additional file 4 of Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid

Supplementary Figure 4: Impact of age and gender on the TF RT-QuIC signal response. The kinetic curves of positive TF RT-QuIC reactions from male and female individuals with prion diseases (PD) were compared across different age groups. Mean fluorescence values from TF RT-QuIC assays were analyzed in female (n = 11; sCJD = 7, FFI = 2, GSS = 1, fCJD [T183A] = 1) and male (n = 11; sCJD = 9, GSS = 1, FFI = 1) prion disease samples (A1). Females were further categorized into younger (50 years, n = 6; sCJD = 6) groups (B1). Males were similarly grouped into younger (39 years, GSS = 1) and older (53–70 years, sCJD = 7, GSS = 1, FFI = 1) groups (C1). The analysis focused on lag phase duration (2) and area under the curve (AUC) values (3). Neither sex (A1–3) nor age group (B1–3, C1–3) showed significant differences in TF RT-QuIC signal responses.