Additional file 6 of Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid

Supplementary Figure 6: RT-QuIC seeding kinetics showing batch-to-batch comparison of recombinant FL Hu E200K substrate. A) Signal responses, seeded with CSF from 6 CJD cases (each 3/3 positive) and 6 non-prion controls (each 0/3 negative), using FL Hu E200K substrate from both batch 1 and batch 2, reveal almost identical kinetic curves. B) Calculation of the area under the curve (AUC) values of sCJD seeded reactions indicated no significant differences between the two batches