[Extended data] Beyond the Beauty: Meristic and Genomic Signatures of Bangka’s Endemic Betta Fishes

This is extended data of the manuscript titled Beyond the Beauty: Meristic and Genomic Signatures of Bangka’s Endemic Betta Fishes.The caption: Suppl Fig 1. The meristic (A) and morphometric (B) characters of Betta spp.Suppl Fig 2. A comparative analysis of the Melanocortin 1 Receptor (MC1R) and Paired box 7 (PAX7) protein sequences among B. burdigala, B. chloropharynx, and B. schalleri, alongside B. splendens, Anabas testudineus, Channa argus, Oreochromis niloticus, Danio rerio, and Carassius auratus. (A) A phylogenetic tree derived from MC1R protein alignments across nine fish species. (B) Multiple Sequence Alignment of the PAX7 MC1R protein in these nine fish species. (C) A phylogenetic tree derived from PAX7 protein alignments across nine fish species. (D) Multiple Sequence Alignment of the PAX7 protein in these nine fish species. The complete protein sequence alignment of MC1R and PAX7 are available in Supplementary file 2 and Supplementary file 3 respectively.Suppl Fig 3. Validation of the CSF1R1 insertion using Oxford Nanopore long-read alignments. Coverage depth across the insertion locus remained consistent (~70–88×), and multiple long reads spanned the insertion region continuously, supporting the authenticity of the predicted insertion and excluding local assembly artifacts.Supplementary file 1. Protein alignment of CSF1R1 in 9 fishes.clustal_numSupplementary file 2. Protein alignment of MC1R in 9 fishes.clustal_numSupplementary file 3. Protein alignment of PAX7 in 9 fishes.clustal_numSupplementary file 4. Coverage depth of CSF1R1_1 region by Oxford Nanopore long-read alignmentsSupplementary file 5. QUAST assembly quality assessment statistics for the three Betta genome assemblies.