Mitochondrial genome evaluation and implications for phylogenetic relationships of Haemadipsa hainana song, Zhang and Tan 1977 (Arhynchobdellida: Haemadipsidae)

Haemadipsa hainana Song, Zhang and Tan 1977, a blood-feeding terrestrial leech, serves as an ecological indicator of habitat quality and potential mammal abundance. Currently, the molecular phylogenetic status of this species remains unclear. In this study, we presented the complete mitochondrial genome of H. hainana for the first time. The assembled mitochondrial genome of H. hainana is 15,800bp in length, comprising 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes and a control region. The nucleotide base content of H. hainana mitogenome was 34.7% A, 42.6% T, 10.7% C, and 12.0% G, indicating a notable AT base bias. Phylogenetic analysis revealed that species of Haemadipsa clustered into one clade, and H. hainana was outside the subclade composed of H. yanyuanensis and H. crenata. These findings will serve as a molecular resource for species identification of H. hainana and contribute valuable information for various genetic, evolutionary, and molecular ecological studies on members of the Haemadipsidae family.