Data for Figures 1-4HGHJ Medicated Serum Regulates BMSC Function via Hippo-YAP Pathway

This dataset supports the core findings of the study titled "Henggu Gushang Heji Medicated Serum Enhances Osteogenic Potential of Rat Bone Marrow Mesenchymal Stem Cells through Modulation of the Hippo-YAP Pathway", focusing on the mechanistic role of a traditional Yi medicine formula (Henggu Gushang Heji, HGHJ) in regulating rat bone marrow mesenchymal stem cell (BMSC) function via the Hippo-YAP signaling pathway. The research falls within the interdisciplinary fields of Stem Cell Research​ and Cell Biology, with data types including Western blot full-membrane images, qRT-PCR raw Ct values, CCK-8 raw OD values, and Transwell migration raw images. 1. Research Background and Scientific Question​ Musculoskeletal disorders (e.g., fractures, osteoarthritis) pose global health challenges, with bone repair dependent on BMSC proliferation, migration, and osteogenic differentiation. HGHJ, a traditional Yi medicine formula documented in the Yunnan Provincial Drug Standards(2005), is clinically used for fracture and osteoarthritis management, but its cellular mechanisms for enhancing bone repair remain unclear. The Hippo-YAP pathway is a key regulator of stem cell behavior and osteogenesis; this study investigates whether HGHJ medicated serum enhances BMSC osteogenic potential by modulating this pathway. 2. Research Objectives​ Validate the effects of HGHJ medicated serum on BMSC proliferation, migration, and osteogenic differentiation. Elucidate HGHJ-mediated regulation of Hippo-YAP pathway components (YAP, p-YAP, LATS2, p-LATS2, CTGF). Determine the necessity of YAP signaling in HGHJ’s effects (via YAP inhibitor Verteporfin). 3. Experimental Design and Dataset Correspondence​ BMSCs were divided into four groups: control (10% blank serum), HGHJ (5% medicated serum), Verteporfin (YAP inhibitor), and combination (HGHJ + Verteporfin). Data were collected via four key experiments, with each dataset corresponding to specific figures and findings: Cell Proliferation (CCK-8 Assay): Raw OD values (including single-well measurements at 24h, 48h, and 72h for all groups) are provided. These data correspond to Figure 1, showing that the HGHJ group exhibited significantly higher OD at 72h (1.1581±0.06) compared to the control group (0.9672±0.05, p