List of species that share identical (100% similarity) 12S ribosomal RNA MiFish-U barcode sequences

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Authors: Matthieu Leray, David Ross Robertson, Gert-Jan Jeunen, Benjamin C Victor
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Abstract

List of species that share identical (100% similarity) 12S ribosomal RNA MiFish-U barcode sequences. Sequences were obtained and compared as follows: 1) Whole mitochondrial genomes were downloaded from the MitoFish database (Sato et al. 2018); 2) Whole mitochondial genomes were imported into the software CRABS (Jeunen G-J et al. 2023); 3) In silico PCR were conducted using the MiFish-U primer set (Miya et al, 2015); 4) The database was dereplicated; 5) A custom python script was used to confirm that different species share the same barcode sequence

Fields of Study & Subjects

Environmental DNA in Biodiversity Studies Fish Biology and Ecology Studies Genomics and Phylogenetic Studies

Publication details

Journal / source
Figshare
Publication date
3 February 2026