Additional file 2 of Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model

Supplementary Material 2. Supplement Fig. 2. Cellular sources and regional expression of BDNF in the mouse brain. A Representative fluorescence images showing the co-localization of BDNF (red) with microglial (IBA1, green), astrocytic (GFAP, green), and neuronal (NeuN, green) markers in the hippocampal formation. Scale bar, 20 μm, 5 μm. B Representative images from a public database showing the expression pattern of Bdnf mRNA in the mouse hippocampal formation, as detected by in situ hybridization (ISH) and Nissl staining (NISS). Scale bar, 100 μm. Data were derived from the Allen Mouse Brain Atlas (mouse.brain-map.org). C Single-cell RNA sequencing data from a public database demonstrating the expression of BDNF in microglia isolated from the mouse hippocampus. Data were obtained from the ABC atlas ( https://abc.sklehabc.com/ ). D A public database demonstrating the expression of BDNF in other brain. ( https://abc.sklehabc.com/ ). E Representative fluorescence images illustrating BDNF (red) and microglial (IBA1, green) co-localization in the hippocampus across different experimental groups. Scale bar, 100 μm. F Quantitative analysis of the co-localization of BDNF with IBA1 in the hippocampus across groups. Data are presented as mean ± SEM (n = 6 mice per group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA with Tukey’s post hoc test)