Additional file 3 of Calcineurin depletion coincides with phosphorylated TDP-43 deposition in a mouse model of ALS/FTLD-TDP

Supplementary Material 3. Figure S3: Hippocampal neurodegeneration in end-stage rNLS8 mouse brain. a–d Representative images of neuronal marker Rbfox3 (NeuN) immunostaining in the hippocampus CA3 region in control animals, and rNLS8 animals at 1 week, 4 weeks, and 7 weeks post-induction. Scale bars: 100 µm. Neuronal density was assessed by measuring NeuN immunoreactivity (e–g) and NeuN positive cells (h–j) in CA3 hippocampal region. Statistical analysis is by unpaired t-test, two-tailed [n = 8 control, 15 rNLS8 animals (1 week); 8 control, 16 rNLS8 animals (4 weeks); 15 control, 15 rNLS8 animals (7 weeks); ns: p > 0.05, **: p < 0.01, ****: p ≤ 0.0001]. All bar graphs represent mean ± SEM.