Parabacteroides distasonis alleviates Clostridioides difficile infection in mice via modulation of secondary bile acids

In this study, we evaluated the preventive and therapeutic potential of P. distasonis against CDI. We examined the inhibitory effects of P. distasonis on C. difficile growth, virulence gene expression, and spore formation in vitro, and assessed its efficacy in CDI mouse models. To elucidate underlying mechanisms, we integrated 16S rRNA gene sequencing and metabolomic profiling to characterize shifts in gut microbial composition and bile acid metabolism.