Additional file 1 of Effect of probiotic supplementation on the gut microbiota diversity in healthy populations: a systematic review and meta-analysis of randomised controlled trials

Additional file1: Table S1 PRISMA checklist 2020. Table S2 Search key. Table S3 Definitions of gut microbiota diversity outcomes reported in the included studies. Supplementary Methods S1 Detailed statistical description of the meta-analysis. Fig. S1 Additional sensitivity analysis for the more restricted analysis of Shannon diversity index. Fig. S2 Additional sensitivity analysis for the subgroups based on risk of bias assessment for Shannon diversity index. Fig. S3 Meta-regression analysis investigating the relationship between intervention duration and the Shannon diversity index. Fig. S4 Additional sensitivity analysis for the more restricted analysis of Observed OTUs. Fig. S5 Additional sensitivity analysis for the subgroups based on risk of bias assessment for Observed OTUs. Fig. S6 Meta-regression analysis investigating the relationship between intervention duration and the number of Observed OTUs. Fig. S7 Additional sensitivity analysis for the more restricted analysis Chao1 index. Fig. S8 Additional sensitivity analysis for the subgroups based on the risk of bias assessment Chao1 index. Fig. S9 Meta-regression analysis investigating the relationship between intervention duration and the Chao1 index. Fig. S10 Additional sensitivity analysis for the more restricted analysis of Simpson’s Index of Diversity. Fig. S11 Additional sensitivity analysis for the subgroups based on risk of bias assessment of Simpson’s Index of Diversity. Fig. S12 Meta-regression analysis investigating the relationship between intervention duration and the Simpson’s Index of Diversity. Fig. S13 Funnel plot to assess publication bias for Shannon diversity index. Fig. S14 Additional leave-one-out analysis for Shannon diversity index. Fig. S15 Funnel plot to assess publication bias for the Observed Operational Taxonomic Units. Fig. S16 Additional leave-one-out analysis for the Observed Operational Taxonomic Units. Fig. S17 Funnel plot to assess publication bias for Chao1 index. Fig. S18 Additional leave-one-out analysis for Chao1 index. Fig. S19 Funnel plot to assess publication bias for Simpson’s Index of Diversity. Fig. S20 Additional leave-one-out analysis Simpson’s Index of Diversity. Table S4 Changes in the microbiome α-diversity indices as measured after the intervention period. Table S5 Changes in the microbiome β-diversity indices as measured after the intervention period. Table S6 Risk of bias assessment for parallel design studies. Table S7 Risk of bias assessment for cross-over design studies Fig. S21 Risk of bias assessment for parallel design studies - Assignment to intervention. Fig. S22 Risk of bias assessment for parallel design studies - Adhering to intervention. Fig. S23 Risk of bias assessment for cross-over design studies - Assignment to intervention. Fig. S24 Risk of bias assessment for crossover design studies - Adhering to intervention. Table S8 GRADE assessment for the meta-analyses of Shannon, Observed OTUs, Chao1 and Simpson’s Index of Diversity indices.