Additional file 2 of Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds

Supplementary Material 2: Fig: 2 Comparison of Lung Composition and Mechanical Properties between Native and Decellularized Mouse Lung Scaffolds. (A) Representative histological images of Masson’s Trichrome, Verhoeff-van Gieson, and Alcian Blue staining demonstrate that decellularization preserves the extracellular matrix composition. Collagen (blue, first row), elastin (black fibers, second row), and sulfated glycosaminoglycans (sGAGs; light blue, third row) show no significant differences between native and decellularized mouse lungs. (B) Uniaxial tensile testing indicates that decellularization does not significantly alter the stress–strain characteristics of the lung scaffold. Solid lines represent the fitted curves, and dashed lines denote the 95% confidence intervals for each group.