Additional file 6 of Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds

Supplementary Material 6: Fig. 6. Reduced apoptosis in anti-CD31 coated re-endothelialized lungs. (A) Representative confocal images showing retention of Caspase 3 staining (red) and DAPI nuclear staining (blue) post whole mouse lung C166 reendothelialization on uncoated (left) and coated (right) whole lung scaffolds. Images are representative of n = 3 biological replicates. Quantification of Caspase 3 staining. Results represent the mean of measurements taken from 3 images per lung seeded with c166 cells from 3 independent experiments. (Each number (n=) represents a biological replicate (re-endothelialized whole lung scaffold). Each group n = 3, mean ± SD, *p < 0.05.