Supplemental Fig. S1 and S2: Krüppel-like factor 2 regulates renin expression in mature juxtaglomerular cells.

Supplemantal data of "Krüppel-like factor 2 regulates renin expression in mature juxtaglomerular cells"Supplemental Fig. S1: RT-qPCR analysis of Klf4 and Klf15 mRNA levels in the kidney cortex of Klf2cKO mice.Klf4 (A) and Klf15 (B) mRNA levels in kidney cortices in Klf2cKO mice (n = 5 males, n = 7 females) and the control groups (n = 7 males, n = 8 females) at 2 months of age. No significant difference was observed in Klf4 and Klf15 expression in both males and females. The Mann-Whitney U test was used to calculate the P-value. All data are shown as individual values with median [IQR]. ns, P ≥ 0.05.Supplementary Fig. S2: Deletion of Klf2 in the renin cell lineage does not show podocyte loss in their glomeruli. A, Immunofluorescent staining for Podocalyxin in kidney sections from Klf2cKO mice (Ren1dCre/+;Klf2fl/fl) and control groups (Ren1d+/+;Klf2fl/fl). Scale bars, 50 μm. B, Quantification of the podocalyxin-positive area per glomerulus (%) revealed no significant difference between Klf2cKO mice and controls at 2 months of age (n = 6 per group; 3 males and 3 females). The Mann-Whitney U test was used to calculate the P-value. ns, P ≥ 0.05. Squares represent male samples and circles represent female samples. Data are shown as individual values with median [IQR].