Additional file 2 of The c-di-AMP binding protein NadD from Mesomycoplasma ovipneumoniae functions as a phosphodiesterase that inhibits host inflammatory responses

Additional file 2. Evaluation of the model of primary sheep alveolar macrophages infected with M. ovipneumoniae. (A) Identification of isolated primary sheep alveolar macrophages using CD14 immunofluorescence. (B) Detection of the M. ovipneumoniae marker gene Hsp70 and the housekeeping gene GAPDH in sheep alveolar macrophages at 0 h, 6 h, 12 h, and 24 h post-M. ovipneumoniae infection. (C) Higher magnification images showing the distribution details of M. ovipneumoniae in sheep alveolar macrophages and the morphology of its inclusions. (D–I) HPLC analysis of c-di-AMP content in sheep alveolar macrophages at 0 h, 3 h, 6 h, 12 h, and 24 h following M. ovipneumoniae infection.